原位雜合在百合育種上應用 In situ hybridization application on the lily breeding

原位雜合在百合育種上應用 In situ hybridization application on the lily breeding 指導老師:金石文 序號:13 摘要 Abstract: 染色體原位雜交技術 (in situ hybridization) 大幅度改良，結合細胞遺傳學、分子生物學、螢光顯微技術及電腦影像處理，發展出新的基因篩檢及定位技術，稱為螢光染色體原位雜交技術 ( Fluorescence in situ Hybridization; 簡 稱FISH )利用螢光標定之DNA 探針，藉由hybridization的過程，在染色體上將基因或DNA定位. 這對於在育種上可以了解該植株的遺傳背景組成是相當方便且快速清晰的方法，能夠很快抓出目標基因，並在染色體上定位出基因片段位置，以原位雜合為輔助，能夠加速育種成效，去了解更多作物的遺傳組成資訊，輔助雜交與回交育種的進行，加速優良性引入新品種 的時間與效率，來達到育成更優良品種。 Keyword: lily, Fluorescent in situ hybridization(FISH),karyotypes,chromosome Genomic in situ hybridization(GISH), REFERENCES: Marasek, A., Hasterok, R., Wiejacha, K. and Orlikowska, T. 2004. Determination by GISH and FISH of hybrid status in ornamental lilies. */ Hereditas 140: 1_/7. Lund, Sweden. ISSN 0018-0661. Received March 20, 2003. AcceptedOctober 30, 2003 Van Creij, M.G.M., Kerckhoffs, D.M.F.J., and Van Tuyl, J.M. 2000. Application of four pollination techniques and of hormone treatment for bypassing interspecific crossing barriers in Lilium L.Acta Hort. 508: 267–274. Van Tuyl, J.M., and Boon, E. 1997. Variation in DNA-content in the genus Lilium. Acta Hort. 430: 829–835.Van Tuyl, J.M., and Lim, K.-B. 2003. Interspecific hybridization and polyploidisation as tools in ornamental plant breeding. Acta Hort. 612: 13–22. Van Tuyl, J.M., and Van Holsteijn, H.C.M. 1996. Lily breeding research in the Netherlands. Acta Hort. 414: 35–46. Van Tuyl, J.M., Van Diën, M.P., Van Creij, M.G.M., Van Kleinwee, T.C.M., Franken, J., and Bino, R.J. 1991. Application of in vitro pollination, ovary culture, ovule culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses. Plant Sci. 74: 115–126. Van Tuyl, J.M., Meijer, B., and Van Dien, M.P. 1992. The use of oryzalin as an alternative for colchicine in in-vitro chromosome doubling of Lilium and Nerine. Acta Hort. 325: 625–630. Van Tuyl, J.M., Lim, K.-B., and Ramanna, M.S. 2002. Interspecific hybridization and introgression.