Murashige & Skoog medium Murashige & Skoog (1962)

Inorganic Salt Formulation

Macronutrients (mg/l)


Ammonium nitrate (NH4NO3)
1,650mg/l
Calcium chloride (CaCl2*H2O)
440mg/l
Magnesium sulfate (MgSO4*7H2O)
370mg/l
Potassium phosphate (KH2PO4)
170mg/l
Potassium nitrate (KNO3)
1,900mg/l

Micronutrients (mg/l)


Boric Acid (H3BO3)
6.2mg/l
Colbalt chloride (CoCl2*6H2O)
0.025mg/l
Cupric Sulfate (CuSO4*5H2O)
0.025mg/l
Ferrous sulfate (FeSO4*7H2O)
27.8mg/l
Manganese sulfate (MnSO4*4H2O)
22.3mg/l
Potassiom Iodine (KI)
0.83mg/l
Sodium molybdate (Na2MoO4*2H2O)
0.25mg/l
Zinc Sulfate (ZnSO4*7H2O)c
8.6mg/l
Na2EDTA*2H2Oa
37.2mg/lb

Common Organic Additives


i-Inositol
100mg/l
Nicotinic Acid
0.5mg/l
Pyridoxine*HCl
0.5mg/l
Thiamine *HCl
0.1mg/l
IAA
1-30mg/l
Kinetin
0.04-10mg/l
Glycine (recrytallized)
2.0g/l
Edamine
1.0g/l
Sucrose
20g/l
Agar
10g/l

a= Originally printed as Na2EDTA
b= Originally printed as 37.3 mg/l anhydrous form
c= Originally printed as ZnSO4*4H2O