Hodgkin and Lyon's Medium ( Hodgkin and Lyon 1986 ) ; standardized for brassica oleracea :

Hodgkin and Lyon's Medium ( Hodgkin and Lyon 1986 ) ; standardized for brassica oleracea :

Socrose 580 mM ( ca. 20% )

Boric acid 1.62 mM ( ca. 100mg/L )

Calcium nitrate 1.69 mM ( ca. 400mg/L )

Magnesium sulfate 0.84 mM ( ca. 200mg/L )

Potassium nitrate 0.99 mM ( ca. 100mg/L )

TAPS 20 mM ( ca. 4.86g/L )

( pH of medium adjusted to 8 with 0.1 N NaOH )